Anti-E Cadherin antibody [EP700Y] – Intercellular Junction Marker

Anti-E Cadherin antibody [EP700Y] – Intercellular Junction Marker

Key features and details

• Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
• Rabbit monoclonal [EP700Y] to E Cadherin – Intercellular Junction Marker
• Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
• Reacts with: Human

• Product name

  Anti-E Cadherin antibody [EP700Y] – Intercellular Junction Marker

• Description

  Rabbit monoclonal [EP700Y] to E Cadherin – Intercellular Junction Marker

• Host species

  Rabbit

• Specificity

  E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. This antibody has been tested on human samples in both WB and IHC. Customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.

• Tested applications

  Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB

• Species reactivity

  Reacts with: Human

• Immunogen

  Synthetic peptide within Human E Cadherin aa 600-700. The exact sequence is proprietary.

• Positive control

  • WB: Positive controls: MCF-7, HT-29, PC-3 whole cell lysate. Negative Control: MDA-MB-231 whole cell lysate and MCF7 whole cell lysate. IHC-P: Human breast carcinoma, lung adenocarcinoma and colonic adenocarcinoma tissue. Human papillary carcinoma of thyroid gland and transitional cell carcinoma of kidney tissue. ICC/IF: MCF7 and HT-29 cells. Flow Cyt (intra): A431 and MCF7 cells.