Antigen Retrieval Buffer (100X TrisEDTA Buffer, pH 9.0) – 125 ml

Antigen Retrieval Buffer (100X TrisEDTA Buffer, pH 9.0) – 125 ml

• Product name

  Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)

• Tested applications

  Suitable for: IHC-P

• General notes

  Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH 9.0) pretreatment.

  This product contains detergent for emulsification of the paraffin.

  1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

  Protocol:

  1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
  2. Wash sections with hot tap water for 1 minute.
  3. Wash sections in buffer for 2×3 minutes.
  4. Continue with IHC protocol