Electrolytic Decalcifying Solution – 500 ml

Electrolytic Decalcifying Solution – 05-M03004 500 ml

Packaging 05-M03004
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500
ml. HDPE cap.
Tamper evident cap.
05-03004Q
Primary container: white bottle in polyethylene terephthalate (PET). Useful capacity 2.5
liters. HDPE cap. Tamper evident cap.
The polyethylenterephthalate is a thermoplastic polymer of the polyester family. PET is an
optimal oxygen, carbon dioxide and other gasses barrier. This material has an high
resistance to ultraviolet radiation and an inertia toward the mainly chemical agents
(solvents: xylene, limonene, liquid paraffines, alcohols, acids, bases etc.). It is biologically
inert. It constitutes a good water and humidity barrier. It shows a great hardness and
mechanical resistance.
The bottle has an optimal grip. The absence of the handles reduces space for storage. The
anti-dropping cap permits a precise and clean use.
Secondary container: carton box.
Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water
and alcohol.
Expected aim Product for the preparation of cyto-histological samples for optical microscopy.
Application Rapid decalcifier, based on hydrochloric and formic acid. It acts on all mineralized tissues:
compact bone, concretions or calcareous deposits in fleshy tissue. A saline corrector has
been added to check tissue swelling caused by acid substances.

Principle At the base of the demineralization process is the chemical reaction between the tissue
calcium (mainly in the form of carbonate, phosphate, oxalate and urate) and hydrochloric
acid and formic acid contained in the decalcifier solution
CaCO3 (insoluble) + 2 HCl = CaCl2 (soluble)+ H2CO3
CaCO3 (insoluble) + HCOOH = Ca(HCOO)2 (soluble) + H2CO3
Method 1) Volume ratio specimen/decalcifier 1 : 100
2) Procedure time 4 – 8 hours – < 5 mm thickness.
3) Post decalcifying procedure: reconditioning of the sample. Rinse with slowly running
water for 1 hour or apply 3 changes of PBS pH 7.4, 20 minutes each.
Components Components CAS CE Index
Hydrochloric Acid 7647-01-0 2315957 017-002-00-2
Formic Acid 64-18-6 2005791 607-001-00-0
Saline Corrector
Deionized water
Warning and
precaution
The product is intended for professional laboratory use for healthcare professionals.
Carefully read the information on the label (danger symbols, risk and safety phrases) and
always consult the safety data sheet. Do not use if the primary container is damaged.
In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.
Storage Store the preparation at room temperature. Keep the containers tightly closed.
Stability After the first opening, the product is reusable until the expiry date, if correctly stored.
Validity: 2 years.
Disposal Hazardous preparation: observe all state and local environmental regulations regarding
waste disposal.