Eosin Y alcoholic solution – 1 L

300,00 AED

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05-M10003 –   500 ml
05-10003 L  –   1 l
05-10003E  –   2.5 l

Packaging – 05-10003E
Primary container: white bottle in polyethylene terephthalate (PET). Useful capacity 2.5 liters. HDPE cap.
Tamper evident cap.
The polyethylenterephthalate is a thermoplastic polymer of the polyester family. PET is an optimal
oxygen, carbon dioxide and other gasses barrier. This material has an high resistance to ultraviolet
radiation and an inertia toward the mainly chemical agents (solvents: xylene, limonene, liquid paraffines,
alcohols, acids, bases etc.). It is biologically inert. It constitutes a good water and humidity barrier. It
shows a great hardness and mechanical resistance.
The bottle has an optimal grip. The absence of the handles reduces space for storage. The anti-dropping
cap permits a precise and clean use.
Secondary container: carton box.
– 05-10003/L
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l. HDPE cap.
Tamper evident cap.
– 05-M10003
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500 ml. HDPE cap.
Tamper evident cap.
Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol.
Expected aim Product for the preparation of cyto-histological samples for optical microscopy.
Application Reagent for cytoplasm staining, excellent for extemporary staining.
Principle The Eosin is an acidic dye that interacts with the cytoplasmic proteins rich in basic amino acids to form a
complex red-pink brilliant .
The Eosin staining (cytoplasmic staining) in combination with the Hematoxylin (nuclear staining ) is the
most used method in the histopathological routine.

Method 1) Hematoxylin-eosin (for routine staining)
Sections to water
Mayer’s hemalum 5 minutes
Tap water (or Scott’s water for 1 minutes) 5 minutes
Eosin y alcoholic solution 1 minutes
Absolute ethanol 1° 30 seconds
Absolute ethanol 2° 30 seconds
Clearing agent and mount
2) Hematoxylin-eosin (for extemporary staining)
Harris’ hematoxylin (or Gill 3) 10 dips
Scott’s water 10 dips
Eosin y alcoholic solution 10 dips
Absolute Ethanol 10 dips (for 3 times)
Clearing agent and mount
Results Nuclei………………………………………………………………………………………………………………………………. purple
Cytoplasm………………………………………………………………………………………………………………………Pink-red
Components Components CAS CE Index
Certified Eosin y 17372- 87-1 241-409-6 –
Ethanol 64-17-5 200-578-6 603-002-00-5
Warning and
precaution
The product must be used exclusively by specialized technical operators.
Carefully read the information on the classification of dangerous substances on the label. Always refer to
the safety data sheet where are available the information on the risks presented by the mixture, the
precautionary measures during use, the measures first aid and the intervention in the event of accidental
release.
Do not use if the primary container is damaged.
Storage Store the preparation at 15-30°C. Keep the containers tightly closed.
Stability After the first opening, the product is usable until the expiry date, if correctly stored. Validity: 2 years.
Disposal Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.

 

 

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Eosin Y alcoholic solution - 1 L

300,00 AED

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