Harris’ Hematoxylin – 1L

05-M06004 –  500 ml
05-06004/L   – 1 l
05-06004E  –  2,5 l

Packaging 05-06004E
Primary container: white bottle in polyethylene terephthalate (PET). Useful capacity 2.5
liters. HDPE cap.
Tamper evident cap.
The polyethylenterephthalate is a thermoplastic polymer of the polyester family. PET is an
optimal oxygen, carbon dioxide and other gasses barrier. This material has an high
resistance to ultraviolet radiation and an inertia toward the mainly chemical agents
(solvents: xylene, limonene, liquid paraffines, alcohols, acids, bases etc.). It is biologically
inert. It constitutes a good water and humidity barrier. It shows a great hardness and
mechanical resistance.
The bottle has an optimal grip. The absence of the handles reduces space for storage. The
anti-dropping cap permits a precise and clean use.
Secondary container: carton box.
05-M06004
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500
ml. HDPE cap. Tamper evident cap.
05-06004/L
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l.
HDPE cap. Tamper evident cap.
Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water
and alcohol.
Expected aim Product for the preparation of cyto-histological samples for optical microscopy.
Application Nuclear staining solution.

Principle

In Harris’ Hematoxylin the active chemical species is the complex formed by hematein(hematoxylin oxidized by potassium iodate) with potassium aluminum sulfate. This
complex has a positive charge and is therefore able to bind to anionic sites present in the
chromatin histone proteins.

Method

1) Sections to distilled water
2) Harris’ hematoxylin, 3 minutes
3) Colour change in tap water (or Scott’s water for 1 minutes), 5 minutes
4) 1% eosin (water solution), 5 minutes
5) Tap water, 5 minutes
6) Dehydrate
7) Clearing agent and mount

Results

Nuclei : purple-blue
Cytoplasm : pink-red

Warning and precaution
The product is intended for professional laboratory use for healthcare professionals.
Carefully read the information on the label (danger symbols, risk and safety phrases) and
always consult the safety data sheet. Do not use if the primary container is damaged.
In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.
Storage Store the preparation at 15-25°C. Keep the containers tightly closed.
Stability After the first opening, the product is reusable until the expiry date, if correctly stored.
Validity: 2 years.
Disposal Hazardous preparation: observe all state and local environmental regulations regarding
waste disposal.