P.A.S. Periodic Acid Schiff according to Hotchkiss – MC Man / 100 tests

04-130802 – P.A.S. Periodic Acid Schiff according to Hotchkiss – MC Man

Product for the preparation of cyto-histological samples for optical microscopy.
To demonstrate normal and pathologic tissue components characterized by adjacent glycolic or aminohydroxylic groups for
histological sections and for Haematology and cytology.
PRINCIPLE
Periodic acid oxidizes selectively the following groups: 1,2 glycolic; primary aminic (1-hydroxy-2-aminic); secondary aminic (1-
hydroxy-2-alkylaminic); 1-hydroxy-2-ketonic. Some methoxyl derivatives and alpha-ketones are oxidized as well, but they are
not converted to aldehydes. During oxidation process the links between carbon atoms in 1,2 position break and consequently
aldehydic groups are formed. In the following reaction, sulphurous fuchsin in Schiff reagent changes these two contiguous
aldehydic groups into an insoluble stained compound similar to basic fuchsin. Three conditions are necessary for these reactions
to take place:
1) Hydroxyl groups must be free,
2) The compounds, which form after oxidation, must not spread in the tissue,
3) There must be enough aldehydic groups in the compounds for a histochemical survey.
Only macromolecules such as glycan and mucins are able to meet these demands. Periodic acid has been chosen as oxidizer
because it arrests oxidation at aldehydic phase. Acid glycan do not react, except for monosulphuric heparin, since the presence
of -SO3H group blocks reactive glycolic groups.
METHOD
METHOD FOR HISTOLOGICAL SECTIONS
1) Bring section to distilled water.
2) Put on the section 10 drops of reagent A: leave to act 10 minutes.
3) Wash in distilled water.
4) Put on the section 10 drops of reagent B: leave to act 20 minutes.
5) Wash in distilled water.
6) Put on the section 10 drops of reagent C: leave to act 2 minutes.
7) Drain the slide and without washing add 10 drops of reagent D: leave to act 2 minutes. Rinse in distilled water.
8) Put on the section 10 drops of reagent E: 3 minutes.
9) Wash in running tap water for 5 minutes.
10)Dehydrate through ascending alcohols. Clear in xylene and mount.
METHOD FOR HEMATOLOGY AND CYTOLOGY
1) Bring section to distilled water.
2) Put on the smear 10 drops of reagent A: leave to act 10 minutes.
3) Wash in distilled water.
4) Put on the smear 10 drops of reagent B: leave to act 15 minutes.
5) Wash in distilled water.
6) Put on the smear 10 drops of reagent C: leave to act 2 minutes.
7) Drain the slide and without washing add 10 drops of reagent D: leave to act 2 minutes. Rinse in distilled water
8) Put on the smear 10 drops of reagent E: 3 minutes.
9) Wash in running tap water for 5 minutes.
10)Dehydrate through ascending alcohols. Clear in xylene mount.