05-12013 – 500 ml
05-12013/L – 1 l
05-12013E – 2,5 l
Packaging – 05-12013E
Primary container: white bottle in polyethylene terephthalate (PET). Useful capacity 2.5
liters. HDPE cap.
Tamper evident cap.
The polyethylenterephthalate is a thermoplastic polymer of the polyester family. PET is an
optimal oxygen, carbon dioxide and other gasses barrier. This material has an high
resistance to ultraviolet radiation and an inertia toward the mainly chemical agents
(solvents: xylene, limonene, liquid paraffines, alcohols, acids, bases etc.). It is biologically
inert. It constitutes a good water and humidity barrier. It shows a great hardness and
The bottle has an optimal grip. The absence of the handles reduces space for storage. The
anti-dropping cap permits a precise and clean use.
Secondary container: carton box.
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l.
HDPE cap. Tamper evident cap.
Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500
ml. HDPE cap. Tamper evident cap.
Expected aim Product for the preparation of: gynecological specimens, urine cytology, fine needle
specimens, sputum and bronchial washings, to be examined by optical microscopy.
Application Cytoplasmic staining for the keratinized cells in the Papanicolaou method.
For the execution of the staining method is required the use of reagents Papanicolaou
Harris hematoxylin and Papanicolaou EA50.
Principle A highly selective blue nuclear stain, Harris’ hematoxylin, is combined with EA50
polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from
eosinophil ones. The last ingredient is OG6 solution, which stains keratinized elements.
1) Ethanol 95°, 2 minutes
2) Distilled water, 2 minutes
3) Harris Hematoxylin, 1 minute
4) Tap water, 5 minutes
5) Ethanol 95°, 15 seconds
6) OG 6, 2 minutes
7) Ethanol 95°, 15 seconds (twice)
8) EA 65, 5 minutes
9) Ethanol 95°, 15 seconds
10) Absolute Ethanol, 30 seconds (twice)
11) Xilene or Bio Clear, 2 minutes (twice)
Results Nuclei : Blue-purple
Cyanophil cytoplasm : Blue-green
Eosinophil cytoplasm : Pink
Keratinized cytoplasm : From pink to orange
The product is intended for professional laboratory use for healthcare professionals.
Carefully read the information on the label (danger symbols, risk and safety phrases) and
always consult the safety data sheet. Do not use if the primary container is damaged.
In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.
Storage Store the preparation at 15-30°C. Keep the containers tightly closed.
Stability After the first opening, the product is reusable until the expiry date, if correctly stored.
Validity: 2 years.
Disposal Hazardous preparation: observe all state and local environmental regulations regarding