Perls meth od for ferric iron  –  100 test

04-180807 –  Perls meth od for ferric iron  –  100 test

Product for the preparation of cyto-histological samples for optical microscopy.
To show reactive ferric iron in tissue sections (method 1) and for haematology and cytology (method 2).
PRINCIPLE
Potassium ferrocyanide reacts with ferric ions of hemosiderin in acid environment to form a coloured salt: Prussian blue.
Reaction takes place in ionic form as follows:
4 Fe++ + 3K4Fe(CN)6 = Fe4(Fe(CN)6)3+ 12 K+
METHOD
1. METHOD FOR HISTOLOGICAL SECTIONS
1) Bring section to distilled water
2) Put on the section 10 drops of reagent A and 10 drops of reagent B: leave to act 20 minutes.
3) Wash well in distilled water.
4) Put on the section 10 drops of reagent C: leave to act 5 minutes.
5) Wash in distilled water
6) Dehydrate in ascending alcohols, clear in xylene and mount.
2. METHOD FOR HAEMATOLOGY AND CYTOLOGY
1) Fix air-dried smears in methanol for 3 minutes. Then air dry again.
2) Put on the section 10 drops of reagent A and 10 drops of reagent B: leave to act 20 minutes.
3) Wash well in distilled water.
4) Cover the smear with reagent C (10-12 drops): leave to act 5 minutes.
5) Wash in distilled water and air dry.